Genetic diversity analysis of Jatropha by Random Amplified Polymorphic DNA (RAPD)

Pattamon Sangin, Sudarat Kasemcholathan


Genetic diversity analysis of Jatropha and intraspecific level of J. curcas were studied using RAPD technique. Six out of one hundred and seven primers produced polymorphic DNA patterns.  A total of 144 bands were scored ranging at 0.25-3.0 kb.  Genetic similarity was estimated by the Jaccard coefficient from NTSYSpc 2.20e version and ranged between 0.46-1.000. A dendrogram was constructed using Unweighted Pair Group Method with Arithmetic Mean (UPGMA) which divided Jatropha into two major clusters. The first group comprised all the 18  J. curcas accessions.  J. curcas (Myanmar), J. curcas (Lumpang) and J. curcas (Chiang Mai) were grouped together with high similarity index (1.000), while J. curcas (Mukdahan) and J. curcas (India) were also clustered together. The second clade included 5 samples of Jatropha as J. gossypifolia, J. podagrica, J. multifida,            J. integerrima (1) and J. integerrima (2), with the latter two clustered together and showing genetic similarity according to their morphological character.

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