Genetic evolution of Oriental wasp, Vespa velutina, in northern of Thailand base on Cytochrome oxidase subunit I DNA barcoding

Touchkanin Jongjitvimol, Sittichai Urtgam

Abstract


The wasp

The Asian predatory wasp, Vespa velutina was found in northern Thailand. Due to the environmental changed which might influence on genetic evolution in insect, therefore, the phylogeny and genetic evolution of this V. velutina is required. This study aimed to investigate the genetic evolution of V. velutina using cytochrome oxidase subunit I (COI) as DNA barcoding comparing to the other species of Vespa spp. The %GC-content of V. velutina’s COI in this study is 33.78% which is higher than that of an average in Vespa group (30.64±1.49%). The Maximum Likelihood tree could categorize Vespa spp. into 4 groups and 9 monophyly. The specimen in northern Thailand was separated from the other V. velutina which was revealed by the very high genetic distance (K2P) of 0.1712. These results can conclude that COI could be used as the powerful DNA barcode for Vespa spp., and COI nucleotide of V. velutina in each locality has highly differenced in genetic evolution which V. velutina in northern Thailand has the highest genetic evolution which mean genetic evolution depends on environmental influence. Moreover, this research could suggest that the V. velutina sampled from northern of Thailand might be another subspecies of V. velutina.

, Vespa velutina was found in northern Thailand. Due to their social behavior, their impact in biological control, and there is one of favored wild food which could affect human health, therefore, the identification and genetic evolution of this Vespa is required. This study is aimed to investigate the genetic evolution of Vespa velutina using cytochrome oxidase subunit I (COI) as DNA barcoding comparing to the other Vespa. The %GC-content of Vespa velutina’s COI in this study is 33.78% which is higher than that of an average in Vespa group (30.64±1.49). The Maximum Likelihood tree could categorize Vespa into 4 groups and 9 monophyly. Vespa velutina specimen in northern Thailand was separated from the other Vespa velutina which revealed a very high genetic distance (K2P) of 0.1712. These results can conclude that COI could be used as the powerful DNA barcode for Vespa, and the genetic evolution of COI in Vespa velutina rapidly changed due to environmental influence. This research could suggest that the Vespa velutina specimen in northern of Thailand might be the subspecies of Vespa velutina.


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