Comparison of the Methods for DNA Extraction from Rhynchostylis spp.
Abstract
Three methods for DNA extraction of Rhynchostylis spp. were examined; Doyle and Doyle
(1987), Lim et. al. (1997) and NucleoSpin?? Plant DNA extraction kit. The results indicated that
DNA isolation from leaf of Rhynchostylis spp. using Doyle and Doyle (1987) method showed no
significant different in quality and quantity of DNA from the NucleoSpin?? Plant DNA extraction
kit. The high quality of DNA from both methods could be confirmed by AFLP analysis and showed better results than Lim???s method when detected by agarose gel electrophoresis and UV absorption
spectrophotometer.
(1987), Lim et. al. (1997) and NucleoSpin?? Plant DNA extraction kit. The results indicated that
DNA isolation from leaf of Rhynchostylis spp. using Doyle and Doyle (1987) method showed no
significant different in quality and quantity of DNA from the NucleoSpin?? Plant DNA extraction
kit. The high quality of DNA from both methods could be confirmed by AFLP analysis and showed better results than Lim???s method when detected by agarose gel electrophoresis and UV absorption
spectrophotometer.
Key words: Extraction methods, DNA, Rhynchostylis spp.
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