DEVELOPMENT FOR PESTICIDE ANALYSIS BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

Abstract

Organochlorine pesticides were used for agricultural pest control but their persistence in
the environment was very long. The most common method for identifying organochlorine pesticides
was gas chromatography that used the complicated sample preparation technique. High performance
liquid chromatography was the another interesting technique that could be used for analysing them.
Determination of 4,4?? DDT and 4,4?? DDE by high performance liquid chromatography were
employed on ??-Bondapak C18 column (3.9x300 mm.) at 226 nm. The organochlorine pesticide
speciations were carried out by using two systems. The first one was the isocratic system that used
65.0% acetonitrile as mobile phase, whereas the last system was the gradient system that varied
acetonitrile concentration from 75.0% to 60.0% within 15 minutes. From the experiment, the
gradient system was found to be more appropriate for separating 4,4?? DDT and 4,4?? DDE. Limits of
detections were 0.12 ppm for 4,4?? DDT and 0.05 ppm for 4,4?? DDE, respectively.

Keywords: 4,4?? DDT/ 4,4?? DDE/ HPLC/ high performance liquid chromatography/ organochlorine pesticides