Comparison in Beta-Glucan Extraction from Mixed Defatted Rice Bran and Khao Dawk Mali Defatted Rice Bran Cultivars Using Taguchi Method of Experimental Design

Pattraporn Phuwadolpaisarn

Abstract


This research aims to compare the extraction of beta-glucan from mixed defatted rice bran and Khao Dow Mali defatted rice bran cultivars by studying factors affecting beta-glucan extraction. Five factors (four levels) were particle size of defatted rice bran (mm) (<75, 75-149, 150-250, non-separated), ratio of water (ml) to defatted rice bran (g) (5:1, 10:1, 15:1, 20:1), pH (4, 7, 8, 10), temperature (??C) (35, 45, 55, 60), and duration?? (h)?? (1, 3, 5, 7). According to the Taguchi method, the experiment was designed as an L16 (45) orthogonal array. The results found that the similarly optimal factors for extracting total beta-glucan from both defatted rice brans were 75-149 mm, 20:1, 55 ??C, and 3 h. However, it has been shown that the optimal pH for both is different. The mixed defatted rice bran prefers to be extracted at pH 4, obtaining a total beta-glucan of 5.26 ?? 0.05 mg/100 g of defatted rice bran. This is less than Khao Dow Mali defatted rice bran cultivars that prefer to extract at pH 10, which obtained a total beta-glucan of 12.35 ?? 0.38 mg/100 g defatted rice bran. Based on the mean S/N ratio, the most influential factors were the ratio of water to defatted rice bran, followed by particle size, pH, temperature, and extraction time.


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References


Abrahamsson, J. (2020). Extraction of ??-glucan from oat bran??? Effect of extraction method and particle size. Swedish: University of Agricultural Sciences.

Asif, A., Faqir, M. A., Tahir, Z., Haq, N., & Zaheer, A. (2010). Extraction and characterization of ??-D-glucan from oat for industrial utilization. International Journal of Biological Macromolecules, 46, 304-309. https://doi.org/10.1016/j.ijbiomac.2010.01.002

Atanasov, J., Schloermann, W., Trautvetter, U., & Glei, M. (2020). The effects of ??-glucans on intestinal health. Ernahrungs Umschau, 67, M140-M147.

Benito-Rom??n, O., Alonso, E., & Lucas, S. (2011). Optimization of the ??-glucan extraction conditions from different waxy barley cultivar. Journal of Cereal Science, 53, 271-276. https://doi.org/10.1016/j.jcs.2011.01.003

Biliaderis, C. G., & Izydorczyk, M. S. (2007). Cereal ??-glucans: Structures, Physical Properties and Physiological Functions. New York: CRC Press.

Chen, H. J., Chang, S. N., & Tang, C. W. (2017). Application of the Taguchi method for optimizing the process parameters of producing lightweight aggregates by incorporating tile grinding sludge with reservoir sediments. Materials (Basel), 10(11), 1294. DOI: 10.3390/ma10111294

Du, B., Bian, Z., & Xu, B. (2014). Skin health promotion effects of natural beta-glucan derived from cereals and microorganisms: a review. Phytotherapy research, 28(2), 159-66. DOI: 10.1002/ptr.4963

Glass, G. E., (2020). Cosmeceuticals: The principles and practice of skin rejuvenation by nonprescription topical therapy. Aesthetic Surgery Journal Open Forum, 112(4), https://doi.org/10.1093/asjof/ojaa038

Idris, F. N., Nadzir, M. M., & Abd Shukor, S. R. (2020). Optimization of solvent-free microwave extraction of Centella asiatica using Taguchi method. Journal of Environmental Chemical Engineering, 8(3), https://doi.org/

1016/j.jece.2020.103766

Inglett, G. E., Carriere, C. J., Maneepun, S., & Tungtrakul, P. (2004). A Soluble fibre gel produced from rice bran and barley flour as a fat replacer in Asian foods. International Journal of Food Science and Technology, 39(1): 1-10. https://doi.org/10.1111/j.1365-2621.2004.00739.x

King Rice Oil Group. (2022). Standard defatted rice bran. https://kingriceoilgroup. com/feeds/defatted-rice-bran-oil/

King Rice Oil Group. (2022). Rice Bran Oil. https://kingriceoilgroup.com/feeds/

defatted-rice-bran-oil/

Lazaridou, A., & Biliaderis, C. G. (2007). Molecular aspects of cereal ??-glucan functionality: physical properties, technological applications and physiological effects. Journal of Cereal Science, 46(2), 101-118.

Limberger-Bayer, V. M., de Francisco, A., Chan, A., Oro, T., Ogliari, P. J., & Barreto, P. L. (2014). Barley ??-glucans extraction and partial characterization. Food Chemistry, 1(154), 84-89. https://doi.org/10.1016/j.jcs.2007.05.003

McCleary, B. V., & Glennie-Holmes, M. (1985). Enzymic quantification of oat (1???3)(1???4)- ??-D-glucan in barley and malt. Journal of Institute of Brewing, 91(5), 285-295.

Megazyme. (2021, January). Mixed-linkage beta-glucan assay procedure (Mccleary method). Retrieved 20 January 2021 from https://www.megazyme.com/ documents/Assay Protocol/K-BGLU_DATA.pdf

Mej??a, S. M. V., de Francisco, A., & Bohrer, B. (2020). A comprehensive review on cereal ??-glucan: extraction, characterization, causes of degradation, and food application, Critical Reviews in Food Science and Nutrition, 60(21), 3693-3704. DOI: 10.1080/10408398.2019.1706444

Michaela, H., & Kraic, J. (2006). Content of beta-D-glucan in cereal grains. Journal of Food and Nutrition Research, 45(3), 97-103.

Mishra, N., & Mishra, N., & Mishra, P. (2020). Influence of different extraction methods on physiochemical and biological properties of ??-glucan from Indian barley varieties. Carpathian Journal of Food Science and Technology, 12, 27-39. DOI:10.34302/crpjfst/2020.12.1.3

Neag, E., Stupar, Z., Varaticeanu, C., Senila, M., & Roman, C. (2022). Optimization of lipid extraction from Spirulina spp. by ultrasound application and mechanical stirring using the Taguchi method of experimental design. Molecules, 27(20), 6794. https://doi.org/10.3390/molecules27206794

Nikoofar, E., Hojjatoleslamy, M., Shakerian, A., Molavi, H., & Shariati, M. A. (2013). Surveying the effect of oat beta glucan as a fat replacer on rheological and physicochemical characteristics of non-fat set yoghurt. International Journal of Farming and Allied Sciences, 2(20), 790-796.

Phuwadolpaisarn, P. (2017). The Influence of conditions on beta-glucan extraction from Thai rice bran cultivars and their biological properties. Proceedings of 95th The IIER International Conference, 8-9 February 2017. Osaka: Japan.

Phuwadolpaisarn, P. (2021). Comparison of ??-glucan content in milled rice, rice husk and rice bran from rice cultivars grown in different locations of Thailand and the relationship between ??-glucan and amylose contents. Molecules, 26, 6368.

DOI: 10.3390/molecules26216368

Salih, G., Alami, R., Jilal, A., & Imani, Y. (2020). Effect of flour particle size on beta-glucan and polyphenol content of three Moroccan oat varieties. Moroccan Journal of Agricultural Sciences, 1 (3), 138-141.

Shoukat, M., & Sorrentino, A. (2021). Cereal ??-glucan: a promising prebiotic polysaccharide and its impact on the gut health. International Journal of Food Science & Technology, 56, 2088-2097. https://doi.org/10.1111/ijfs.14971

Surin Bran Oil. (2022). Process: Extraction and Proprietary Refining. https://surinbranoil.co.th/

Szpicer, A., Onopiuk, A., P????torak, A., & Wierzbicka, A. (2020). The influence of oat ??-glucan content on the physicochemical and sensory properties of low-fat beef burgers. CyTA - Journal of Food, 18(1), 315-327.

Ul Ain, H. B., Saeed, F., Khan, M. A., Niaz, B., Tufail, T., Anjum, F. M., Hussain, S., & Madiha, R. (2019). Isolation and characterization of cereal cell walls. International Journal of Food Properties, 22(1), 130-137. DOI:10.1080/19476337.2020.1750095

Vasanthan, T., & Temelli, F. (2008). Grain fractionation technologies for cereal beta-glucan concentration. Food Research International, 41(9), 876-881.

https://doi.org/10.1016/j.foodres.2008.07.022

Zhu, F., Du, B., & Xu, B. (2016). A critical review on production and industrial applications of beta-glucans. Food Hydrocolloids, 52, 275-288. https://doi.org/10.1016/j.foodhyd.2015.07.003


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