Gametophytic Morphology of Platycerium holttumii Jonch. & Hennipm. in vitro

Abstract

An investigation of the morphological characters and gametophytic developmental stages of
Platycerium holttumii Jonch. & Hennipm. In vitro were observed. In vitro spore culture of
Platycerium holttumii Jonch. & Hennipm. on Miller & Miller (MM) (1961) medium was also
performed and investigated. The results found that spores germinated within 2 weeks. At 5 weeks
after germination spore grew up to the protonema stage and developed to prothallus within 7 weeks and then became mature prothallus after 10 weeks of culture. Antheridium and archegonium could
be observed after 14 and 16 weeks of culture, respectively. The fertilization between eggs and
sperms was noticed at 22 weeks and the embryo grew into sporophyte after 26 weeks of culture.

Keywords: Platycerium holttumii Jonch. & Hennipm., Gametophytic morphology, In vitro