Effect of UV and EMS mutagenesis on xylanase production of Streptomyces thermocarboxydus ME 742 for oil palm waste composting
Abstract
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Composting is one of most favored alternative to manage the large quantity of wastes generated from palm oil mill including empty fruit bunches (EFB) and decanter cake (DC) to avoid environmental pollutions. Microorganism is one of factor determinant for the degradation rate of raw material, therefore applying superior microorganism in process is a promising approach to enhance composting efficiency.?? In this study, the main aim was to improve xylanase production by Streptomyces thermocarboxydus ME742, which had been classified as thermotolerant lignocellulolytic bacteria and can be used in compost preparation. Firstly, UV exposure was applied to increase the production of lignocellulolytic enzyme, mainly xylanase. Unfortunately, this method could not induce a significant increase in xylanase production when mixed substrate of EFB and DC was used as nutrient for microbial growth, even they showed clear zone on xylan-containing agar about 10-13 cm bigger than that of wild-type.?? Mutants with enhanced 0.05-0.23 fold in xylanase production were obtained by EMS treatment for 60 min at concentration of 15 ??g/mL. Through cultivation for 5 generations, 8 mutants possessed significantly increase in their xylanase production reached to 1.95-2.06 fold higher than wild type. Among these mutants, MEEMS16 was most genetically stable mutant which produced xylanase at 704.90 ?? 21.91 U/g substrate, accounted to 2.06 fold of wild type. This study indicates that EMS was an effective mutagenic agent to generate mutation of S. thermocarboxydus ME742 with promising xylanase activity for use in decomposition of EFB and DC.
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Abdel-Aziz, M. S., Talkhan, F. M., Fadel, M., Abouzied, A. A. & Abdel-Razik, A.S. (2011). Improvement of xylanase production from Streptomyces pseudogriseolus via UV Mutagenesis. Australian Journal of Basic and Applied Sciences, 5(5), 1045-1050.https://www.researchgate.net/publication/282321317_Improvement_of_xylanase_production_from_Streptomyces_Pseudogriseolus_via_UV_mutagenesis
Adam, S. Syd Ahmad, SS., Hamzah, N.M. & binit Darus, N.A. (2014). Composting of empty fruit bunch treated with palm oil mill effluent and decanter cake. in book: Regional Conference on Science, Technology and Social Sciences, 437-445. https://www.researchgate.net/publication/313797393_Composting_of_Empty_Fruit_Bunch_Treated_with_Palm_Oil_Mill_Effluent_and_Decanter_Cake
Cherypiew, N., Tachapattaworakul. & Suksaroj, T. (2014). Waste management in oil palm industry: A case study of co-composting of oil palm empty fruit bunches and decanter cake slurry. Proceedings of Fifth TheIIER-SCIENCE PLUS International Conference, Singapore. 31-35. https://www.worldresearchlibrary.org/up_proc/pdf/2-141905927931-35.pdf
Dashtban, M., Buchkowski, R., Qin, W. (2011). Effect of different carbon sources
on cellulose production by Hypocrea jecorina (Trichoderma reesei) strains. International Journal of Biochemistry and Molecular Biology, 2, 274-286. https://www.ijbmb.org /ISSN:2152-4114/IJBMB1108001
Dayana Amira, R., Roshanida, A.R. & Rosli, M.I. (2012). Effects of xylanase and cellulase production during composting of EFB and POME using fungi. World Academy of Science, Engineering and Technology. International Journal of Biotechnology and Bioengineering, 6(8), 581-584.
https://doi.org/10.5281/zenodo.1332930
Duangwang, S., & Sangwichien, C. (2015). Utilization of oil palm empty fruit bunch hydrolysate for ethanol production by Baker???s Yeast and Loog-Pang. Energy Procedia, 79, 157-162. https://doi.org/10.1016/j.egypro.2015.11.455
Fauzianto, R. (2014). Implementation of bioenergy from palm oil waste in Indonesia. Journal of Sustainable Development Studies, 5, 100-115.
https://www.researchgate.net/publication/270507891_Implementation_of_Bioenergy_from_Palm_Oil_Waste_in_Indonesia
Jafri, N., Malmiri, H.J., Mivehroud, M.H., & Adibpour, M. (2017). Optimization of UV irradiation mutation conditions for cellulase production by mutant fungal strains of Aspergillus niger through solid state fermentation. Green Processing and Synthesis, 6, 333-340. https://doi.org/10.1515/gps-2016-0145
Kananama, W., Tachapattaworakul Suksaroja, T. & Suksaroj, C. (2011). Biochemical changes during oil palm (Elaeis guineensis) empty fruit bunches composting with decanter sludge and chicken manure. Science Asia, 37, 17-23.
https://10.2306/scienceasia1513-1874.2011.37.017
Kavitha, B., Jothimani, P. and Rajannan, G. (2013). Empty fruit bunch-A Potential organic manure for agriculture. International Journal of Science, Environment and Technology, 2 (5), 930-937. https://www.ijset.net/journal/167.pdf
Khangkhachit, W., Suyotha, W., O-Thong, S. & Prasertsan, P. (2021). Selection of microorganisms possessing thermostable lignocellulolytic enzymes and application of the enzymes for saccharifcation of pretreated palm oil mill wastes. Waste and Biomass Valorization, 12, 711???724. https://doi.org/10.1007/s12649-020-01027-z
Kumar, S.K., Manimaran, A., Permaul, K., & Singh, S. (2009). Production of ??-xylanase by a Thermomyces lanuginosus MC 134 mutant on corn cobs & its application in biobleaching of bagasse pulp. Journal of Bioscience and Bioengineering, 107(5), 494-498. https://10.1016/j.jbiosc.2008.12.020
Kumar A.K. (2015). UV mutagenesis treatment for improved production of endoglucanase & ??-glucosidase from newly isolated thermotolerant actinomycetes, Streptomyces griseoaurantiacus. Bioresour, Bioprocess, 2, 22. https://10.1186/s40643-015-0052-x
Miller GL. (1959). Use of dinitrosalicylic acid reagent for determination of reducing sugar. Analytical chemistry, 31, 426-428. https://doi.org/10.1021/ac60147a030
Nutongkaew, T., Duangsuwan, W., Prasertsan, S. & Prasertsan, P. (2011). Production of compost from palm oil mill biogas sludge mixed with palm oil mill wastes and biogas effluent. Proceedings of the Thailand Chemical Engineering and Applied Chemistry Conference (TIChE) International Conference, 10-11, 1-5. https://chem.eng.psu.ac.th/tiche2011/TCHE/data/paper/international/fb/oral/fb106.pdf
Nutongkaew, T., Duangsuwan, W., Prasertsan, S. & Prasertsan, P. (2014). Effect of inoculum size onproduction of compost and enzymes from palm oil mill biogas sludge mixed with shredded palm empty fruit bunches and decanter cake. Songklanakarin Journal of Science and Technology, 36 (3), 275-281. https://rdo.psu.ac.th/sjstweb/journal/36-3/36-3-4.pdf
Porsuk I, zakin S, Bali B, In e Yilmaz E. (2013). A cellulase-free, thermo active, and alkali xylanase production by terrestrial Streptomyces sp. CA24. Turkish Journal of Biology, 37, 370???375. https://10.3906/biy-1208-40
Tasneem M, Khan A, Ashraf H, Haq I. 2003. Xylanase biosynthesis by chemically mutated strain of Aspergillus niger. Journal of Food Technology, 1, 178-181. http://docsdrive.com/pdfs/medwelljournals/jftech/2003/178-181.pdf
Wattanasit, K., Asawatreratanakul, K. & O-thong, S. (2013). Potential of biogas production of oil palmmill residues and its anaerobic co-digestion under thermophilic condition. Thaksin University Journal, 16(3), 48-58. http://10.1016/j.egypro.2017.10.206
Yahya, A., Sye, C.P., Ishola, T.A. & Suryanto, H. (2010). Effect of adding palm oil mill decanter cakes slurry with regular turning operation on the composting process and quality of compost from oil palm empty fruit bunches. Bioresource Technology, 101, 736???8741. https://doi.org/10.1016/j.biortech.2010.05.073