Micropropagation of Musa (AA) 'Kluai Khai' Using Temporary Immersion Bioreactor System (TIBs)
Abstract
The factors affected by micro-propagated banana, Musa (AA) ???Kluai Khai??? using a temporary immersion system were investigated. Suckers of good quality mother plants were obtained from a banana conservation farm. ???Kluai Khai??? is a geographic indication plant of Kamphaeng Phet Province Thailand. Excised shoot tips were used to initiate shoot on semi-solid MS medium supplemented with 3 mg/L of BA. Shoot multiplication was done through subcultures every month intervals. Small shoot buds of the fourth subculture were transferred to liquid MS medium supplemented with 5 mg/L of BA and cultivated for 5 weeks. Optimization conditions TIB production system was determined by varied shoot density, media volume, time, and frequency of immersion. An experiment was set into 16 treatments. Data were collected during 35 days of cultivation in TIB glasses vessel size 2000 ml. The optimized condition of cultivation of Khluai Khai in TIB was found when using 10 shoots per reactor, immersion time for 10 minutes for every 12 hours a day, and medium volume at 600 ml. The maximum plantlets (4.19 ?? 0.68/explant) with a 91.41 % of survival rate were obtained. An assessment of genetic fidelity was performed using a flow cytometer. Results revealed that both the mother plant and TIBs??? plantlets were diploid (2n). ??
Full Text:
PDFReferences
Ahmadian M, Babaei A, Shokri S,and Hessami S,. (2017). Micropropagation of carnation (Dianthus caryophyllus L.) in liquid medium by temporary immersion bioreactor in comparison with solid cultres. Journal of Genetic Engineering and Biotechnology, 15, 309-315.
Bukar Y, A., Muhammad HS., and Lucy JO. (2017). Installation, Operation and Sustainability of the Temporary Immersion Bioreactor System. Journal of Scientific and Engineering Research, 4(4):259-265.
Etienne H, and Berthouly M.2002.Temporary immersion system in plant micropropagation. Plant Cell, Tissue and Organ Culture 69:215-231.
Gonzalez R, Rios D, Aviles F, Sanchez-Olate M. (2011). Multiplication in vitro de Eucalyptus globulus mediate Sistema de immersion temporal Bosque, 32(2):147-154.
Frometa O, M, Morgado M,E,M, Silva J. A, T, Morgado D,T,P, Gradaille M, A, D,.(2017). In vitro propagation of Gerbera jamesonii Bolus ex Hooker f. in a temporary immersion bioreactor. Plant Cell Tiss Organ Cult, 129:543-551.
Kunaknonnuruk B, Inthima P, Kongbangkerd A,. (2019). In Vitro Propagation of Rheophytic Orchid, Epipactis flava Seidenf.???A Comparison of Semi-Solid, Continuous Immersion and Temporary Immersion Systems. Biology, 8(4), 72; https://doi.org/10.3390/biology8040072.
Lyam P. T, Mutah L.M, Zainab OJ,.2012. The potential of Temporary Immersion Bioreactors (TIBs) in Meeting Crop Production Demand in Nigeria. Journal of Biology and Life Science, 3(1):66-86.
Ma & Huang. (1982). First report of plantain leaf spot casual by Pestlotiops ismenacesiaua in China the European Physical.
Marbun C,L,M, Touruan-Mathius N, Utomo C, Liwang T,.(2015). Micropropagation of Embryogenic Callus of
Oil Palm ( Elaeis guineensis Jacq.) Using Temporary Immersion System. Procedia Chemistry 14, 122-129.
Nwauzoma A, B, and Jaja E,T,. (2013). A Review of somaclonal variation in Plantain (Musa ssp.): mechanisms and applications. Journal of Applied Biosciences, 67, 5252-5260.
Zhang B, Song L, Bekele LD, Shi J, Jia Qi, Zhang B, Jin L, Duns GJ, Chen J. (2018). Optimizing factors affecting development and propagation of Bletilla striata in temporary immersion bioreactor system. Scientia Horticulturae 232, 121-126.
Zou, N, Li Y., Lain F., 2013. Tissue culture and rapid propagation of excellent ormamental and medicinal ground cover plants Bletilla atriata. Acta Agric. Univ. Jiangxiensis 35, 950-955.
Refbacks
- There are currently no refbacks.