Micropropagation of Musa (AA) 'Kluai Khai' Using Temporary Immersion Bioreactor System (TIBs)

siripong - premjet, Duangporn Premjet, Wimonmas Kunkaew, Anupan Kongbangkerd, Siripong Premjet

Abstract


The factors affected by micro-propagated banana, Musa (AA) ???Kluai Khai??? using a temporary immersion system were investigated. Suckers of good quality mother plants were obtained from a banana conservation farm. ???Kluai Khai??? is a geographic indication plant of Kamphaeng Phet Province Thailand. Excised shoot tips were used to initiate shoot on semi-solid MS medium supplemented with 3 mg/L of BA. Shoot multiplication was done through subcultures every month intervals. Small shoot buds of the fourth subculture were transferred to liquid MS medium supplemented with 5 mg/L of BA and cultivated for 5 weeks. Optimization conditions TIB production system was determined by varied shoot density, media volume, time, and frequency of immersion. An experiment was set into 16 treatments. Data were collected during 35 days of cultivation in TIB glasses vessel size 2000 ml. The optimized condition of cultivation of Khluai Khai in TIB was found when using 10 shoots per reactor, immersion time for 10 minutes for every 12 hours a day, and medium volume at 600 ml. The maximum plantlets (4.19 ?? 0.68/explant) with a 91.41 % of survival rate were obtained. An assessment of genetic fidelity was performed using a flow cytometer. Results revealed that both the mother plant and TIBs??? plantlets were diploid (2n). ??


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